Characterization and comparative analysis of 2,4-toluene diisocyanate and 1,6-hexamethylene diisocyanate haptenated human serum albumin and hemoglobin

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Characterization and comparative analysis of 2,4-toluene diisocyanate and 1,6-hexamethylene diisocyanate haptenated human serum albumin and hemoglobin.

Diisocyanates (dNCOs) are low molecular weight chemical sensitizers that react with autologous proteins to produce neoantigens. dNCO-haptenated proteins have been used as immunogens for generation of dNCO-specific antibodies and as antigens to screen for dNCO-specific antibodies in exposed individuals. Detection of dNCO-specific antibodies in exposed individuals for diagnosis of dNCO asthma has...

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Quantitation of 4,4′-methylene diphenyl diisocyanate human serum albumin adducts

4,4'-Methylene diphenyl diisocyanate (herein 4,4'-MDI) is used in the production of polyurethane foams, elastomers, coatings, adhesives and the like for a wide range of commercial products. Occupational exposure to MDI levels above current airborne exposure limits can elicit immune mediated hypersensitivity reactions such as occupational asthma in sensitive individuals. To accurately determine ...

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Cytokine secretion patterns of T cells responding to haptenized-human serum albumin in toluene diisocyanate (TDI)-induced asthma patients.

Our understanding immune response mechanisms to chemical allergens has been limited. It was partly due to the nature of antigens, recognized by T cells, not being well characterized. In the present study, we examined a hypothesis that a reactive chemical allergen, toluene diisocyanate (TDI), react with autologous proteins, thereby inducing T cell responses to the modified self protein in vivo. ...

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Longitudinal study of specific antibodies to toluene diisocyanate (TDI)-human serum albumin (HSA) conjugate in patients with TDI-induced asthma

BACKGROUND An appreciable number of patients with toluene diisocyanate (TDI)-induced asthma have high serum levels of specific IgE (sIgE) antibody to TDI-human serum albumin conjugate (HSA). A recent investigation suggested a role of specific IgG (sIgG) in the development of TDI asthma. METHODS We observed the changes in the levels of specific IgE and IgG antibodies to TDIHSA conjugate in TDI...

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ژورنال

عنوان ژورنال: Journal of Immunological Methods

سال: 2016

ISSN: 0022-1759

DOI: 10.1016/j.jim.2016.02.005